In Silico Anaysis of Gene Sequence Variation and Molecular Characterization Segregation and Condensation Protein B (ScpB) Streptococcus agalactiae

Abstract

Streptococcus agalactiae is a causes sepsis in mothers and neonates. Cases of infection in the United States are estimated at 8000 cases of GBS infection early – onset occurs annually in cases per 1000 live births. Approximately 4-6% of infected babies die and 25-50% survive will experience sequelae such as mental retardation, blindness and deafness. Gold standard for diagnosing sepsis is the culture method, but this method has limitations in the examination, including requiring a long time, the culture results which are declared negative after being confirmed by the PCR method get positive results. The result of culture examination was not infected and when the baby was born get suffered from sepsis. The ScpB gene plays a role in immunogenic factors. Therefore, this study aims to molecularly characterizing variants in the ScpB gene of Streptococcus agalactiae back bacteria. Genetic variations in a population will influence traits and responses. This research aims to analyze genetic variations in the ScpB sequence Streptococcus agalactiae NCBI with PopSet 2572585596 using BsmAI restriction enzyme with RFLP method in silico. The results showed that there were genetic variations in the ScpB gene sequence and three allelic variations contained in 10 sequences. ScpB is also responsible for the response to antibiotics. Some antibiotics are resistance, even the antibiotic recommended by. FDA omandacycyline, is also caused by. the biofilm which product from ScpB. In conclusion, there are 3 allelic variations result from in silico RFLP, these variations will certainly have varying effect including immune responses, biofilm production and resistance to antibiotics.